杭州浙大迪迅生物基因工程有限公司Int Arch Allergy Immunol：无症状尘螨致敏和尘螨诱导变应性鼻炎患者的IgE和IgG4谱
发布日期：2023-01-22
原标题：无症状尘螨致敏和尘螨诱导变应性鼻炎患者的IgE和IgG4谱
方法：本研究共纳入48例对HDM致敏的受试者：27例伴哮喘（症状组），21例无过敏症状（无症状组）。6名健康人为对照组。采集外周血标本进行浙大迪迅尘螨组分血清IgE和IgG4检测和嗜碱性粒细胞活化试验(BATs)。IgE和IgG4检测针对尘螨组分Der p 1、Der p 2、Der p 7、Der p 10、Der p 21、Der p 23和Der f 1、Der f 2。
结果：与无症状受试者相比，AR患者的SPT风团直径更大（7.0比3.0 mm，p < 0.0001），对 Der p的特异性IgE更高（15.50比0.70 KU/L，p < 0.0001）。两组都表现出对 Der p 1 和 Der p 2 更频繁的敏感性（p < 0.05）。但两组间总IgE和特异性IgG4差异无统计学意义。在AR患者中观察到用HDM刺激后的嗜碱性粒细胞活化反应更高（所有p < 0.05）。
结论：与对HDM敏感的无症状受试者相比，AR患者在SPT，屋尘螨血清特异性IgE，尘螨组分Der p 1和Der p 2水平以及BAT上存在差异。单独的尘螨组分特异性IgG4不能区分无症状个体和AR患者。
原始出处
Int Arch Allergy Immunol
DOI: 10.1159/000517824
Abstract:
Introduction: Asymptomatic sensitization is defined as the presence of positive skin prick test (SPT) and/or positive serum allergen-specific IgE in the absence of clinical allergic symptoms. Currently, there is no convincing explanation why some people with positive allergen tests do not show symptoms. We aimed to investigate the house dust mite (HDM)-specific IgE and IgG4 repertoire in asymptomatic HDM-sensitized subjects and HDM-induced allergic rhinitis(AR) patients.
Methods: A total of 48 subjects sensitized to HDM were included in this study: 27 had AR with/without asthma (symptomatic group), and 21 had no allergic symptoms(asymptomatic group). Six healthy individuals served as control group. Peripheral blood samples were collected for serum IgE and IgG4 assay and basophil activation tests(BATs). IgE and IgG4 assay included antibodies to Dermatophagoides(Der) p1, 2, 7, 10, 21, 23, and Der f1, 2.
Results: AR patients had a larger wheal diameter of SPT (7.0 vs. 3.0 mm,p < 0.0001) and a higher specific IgE to Der p (15.50 vs. 0.70 KU/L, p < 0.0001) than asymptomatic subjects. They also showed more frequent sensitization to Der p1 and Der p2 (both p < 0.05). However, the total IgE and specific IgG4 did not differ significantly between the 2 groups. The basophil activation response after being stimulated with HDM was observed to be higher in AR patients (all p < 0.05).
Conclusions: There are differences in SPT, serum-specific IgE to Der p, component allergen Der p1 and Der p2 level and BAT between AR patients and asymptomatic subjects sensitized to HDM. IgG4 alone cannot differentiate asymptomatic individuals from AR patients.
First Author:
Qingxiu Xu
Correspondence:
Rongfei Zhu, zrf13092 @ 163.com
2023-01-13 Article
——浙大迪迅译
背景：无症状致敏的定义为在没有临床过敏症状的情况下，存在皮肤点刺试验(SPT)阳性和/或血清过敏原特异性IgE阳性。目前，还没有确切解释来说明为什么一些过敏原检测呈阳性的人没有出现症状。我们本次研究的目的是调查无症状尘螨（HDM）致敏受试者和HDM诱导的变应性鼻炎(AR)患者的HDM特异性IgE和IgG4谱。方法：本研究共纳入48例对HDM致敏的受试者：27例伴哮喘（症状组），21例无过敏症状（无症状组）。6名健康人为对照组。采集外周血标本进行浙大迪迅尘螨组分血清IgE和IgG4检测和嗜碱性粒细胞活化试验(BATs)。IgE和IgG4检测针对尘螨组分Der p 1、Der p 2、Der p 7、Der p 10、Der p 21、Der p 23和Der f 1、Der f 2。
结果：与无症状受试者相比，AR患者的SPT风团直径更大（7.0比3.0 mm，p < 0.0001），对 Der p的特异性IgE更高（15.50比0.70 KU/L，p < 0.0001）。两组都表现出对 Der p 1 和 Der p 2 更频繁的敏感性（p < 0.05）。但两组间总IgE和特异性IgG4差异无统计学意义。在AR患者中观察到用HDM刺激后的嗜碱性粒细胞活化反应更高（所有p < 0.05）。
结论：与对HDM敏感的无症状受试者相比，AR患者在SPT，屋尘螨血清特异性IgE，尘螨组分Der p 1和Der p 2水平以及BAT上存在差异。单独的尘螨组分特异性IgG4不能区分无症状个体和AR患者。
原始出处
Int Arch Allergy Immunol
[IF：3.767]
IgE and IgG4 Repertoire in Asymptomatic HDM-Sensitized and HDM-Induced Allergic Rhinitis PatientsDOI: 10.1159/000517824
Abstract:
Introduction: Asymptomatic sensitization is defined as the presence of positive skin prick test (SPT) and/or positive serum allergen-specific IgE in the absence of clinical allergic symptoms. Currently, there is no convincing explanation why some people with positive allergen tests do not show symptoms. We aimed to investigate the house dust mite (HDM)-specific IgE and IgG4 repertoire in asymptomatic HDM-sensitized subjects and HDM-induced allergic rhinitis(AR) patients.
Methods: A total of 48 subjects sensitized to HDM were included in this study: 27 had AR with/without asthma (symptomatic group), and 21 had no allergic symptoms(asymptomatic group). Six healthy individuals served as control group. Peripheral blood samples were collected for serum IgE and IgG4 assay and basophil activation tests(BATs). IgE and IgG4 assay included antibodies to Dermatophagoides(Der) p1, 2, 7, 10, 21, 23, and Der f1, 2.
Results: AR patients had a larger wheal diameter of SPT (7.0 vs. 3.0 mm,p < 0.0001) and a higher specific IgE to Der p (15.50 vs. 0.70 KU/L, p < 0.0001) than asymptomatic subjects. They also showed more frequent sensitization to Der p1 and Der p2 (both p < 0.05). However, the total IgE and specific IgG4 did not differ significantly between the 2 groups. The basophil activation response after being stimulated with HDM was observed to be higher in AR patients (all p < 0.05).
Conclusions: There are differences in SPT, serum-specific IgE to Der p, component allergen Der p1 and Der p2 level and BAT between AR patients and asymptomatic subjects sensitized to HDM. IgG4 alone cannot differentiate asymptomatic individuals from AR patients.
First Author:
Qingxiu Xu
Correspondence:
Rongfei Zhu, zrf13092 @ 163.com
2023-01-13 Article
