鱼类过敏原的诊断测试受限于大量未研究的鱼类。四种鲑鱼过敏原是特征明确的，并在WHO/IUIS 进行了注册，而迄今为止尚未描述过鲇鱼过敏原。2008年，淡水养殖鲇鱼的产量超过了全球养殖最多的海洋鱼类鲑鱼。本研究旨在识别、量化和比较鲑鱼和鲇鱼中的所有 IgE 结合蛋白。
与鲑鱼相比，鲇鱼的生肉提取物和熟肉提取物显示出更高的IgE结合频率（分别为77%对70% 和64%对53%）。主要鱼类过敏原小清蛋白显示出最高的IgE结合能力 (10%-49%)，其次是生肉中的磷酸丙糖异构酶 (TPI；19%-34%) 和熟肉提取物中的原肌球蛋白 (6%-32%)。包括TPI在内的六种以前未识别的鱼类过敏原已在 WHO/IUIS 注册。分别有14%和10%的患者通过IgE检测到来自鲑鱼和鲇鱼的肌酸激酶。鲇鱼L-乳酸脱氢酶、3-磷酸甘油醛脱氢酶、丙酮酸激酶和葡萄糖-6-磷酸异构酶显示出会与6%-13%的患者出现IgE 结合。在鲑鱼中，这些蛋白质无法成功分离。
Expanding the allergen repertoire of salmon and catfish
Diagnostic tests for fish allergy are hampered by the large number of under-investigated fish species. Four salmon allergens are well-characterized and registered with the WHO/IUIS while no catfish allergens have been described so far. In 2008, freshwater-cultured catfish production surpassed that of salmon, the globally most-cultured marine species. We aimed to identify, quantify, and compare all IgE-binding proteins in salmon and catfish.
Seventy-seven pediatric patients with clinically confirmed fish allergy underwent skin prick tests to salmon and catfish. The allergen repertoire of raw and heated protein extracts was evaluated by immunoblotting using five allergen-specific antibodies and patients' serum followed by mass spectrometric analyses.
Raw and heated extracts from catfish displayed a higher frequency of IgE-binding compared to those from salmon (77% vs 70% and 64% vs 53%, respectively). The major fish allergen parvalbumin demonstrated the highest IgE-binding capacity (10%-49%), followed by triosephosphate isomerase (TPI; 19%-34%) in raw and tropomyosin (6%-32%) in heated extracts. Six previously unidentified fish allergens, including TPI, were registered with the WHO/IUIS. Creatine kinase from salmon and catfish was detected by IgE from 14% and 10% of patients, respectively. Catfish L-lactate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, and glucose-6-phosphate isomerase showed IgE-binding for 6%-13% of patients. In salmon, these proteins could not be separated successfully.
We detail the allergen repertoire of two highly farmed fish species. IgE-binding to fish tropomyosins and TPIs was demonstrated for the first time in a large patient cohort. Tropomyosins, in addition to parvalbumins, should be considered for urgently needed improved fish allergy diagnostics.
Andreas L. Lopata; Pharmacy and Medical Research, Bldg. 47,1 James Cook Drive, James Cook University, Townsville, QLD 4811, Australia
Thimo Ruethers, Aya C. Taki, Shaymaviswanathan Karnaneedi, Shuai Nie, Tanja Kalic, Danyi Dai, Sakda Daduang, Michael Leeming, Nicholas A. Williamson, Heimo Breiteneder, Sam S. Mehr, Sandip D. Kamath, Dianne E. Campbell, Andreas L. Lopata