杭州浙大迪迅生物基因工程有限公司

原标题：尽管与Ara h6相似，Ara h2是仍是花生的主要过敏原
　　①背景：Ara h 2特异性IgE (sIgE)是目前诊断花生过敏的最佳血清学指标。Ara h 6与Ara h 2共享60%的序列特性和多个表位。②目的：评价Ara h 2和Ara h 6对花生过敏的诊断价值和相对重要性。③方法：研究了花生过敏(PA)、花生致敏但耐受(PS)和非致敏非过敏(NA)儿童队列(n=100)。花生和个别过敏原的sIgE水平采用 ImmunoCAP定量。分别对Ara h 2和Ara h 6进行ImmunoCAP 抑制实验和肥大细胞活化试验(MAT)。采用SPSS v14和Prism v7进行统计分析。④结果：Ara h 2 -sIgE和Ara h 6 -sIgE对花生过敏的诊断准确性高于花生提取物和其他花生过敏原组分sIgE的诊断准确性。大部分PA患者对Ara h 2和Ara h 6均敏感。Ara h2对Ara h 2 - sIgE结合的抑制作用大于Ara h 6 (p<0.001)，而Ara h 2和Ara h 6对Ara h 6 - sIgE结合的抑制作用相似(p=0.432)。在MAT上，当检测共敏个体时，Ara h 2诱导的最大反应活性(p=0.001)明显高于Ara h 6 而EC50 (p=0.002)明显低于Ara h 6 。⑤结论：Ara h 2 - sIgE和Ara h 6 - sIgE诊断花生过敏的准确性最高。在英国花生过敏中，尽管Ara h 2与Ara h 6存在一定程度的交叉反应，但Ara h 2仍是主要的花生过敏蛋白。


延伸阅读
JACI                                                  
Ara h 2 is the dominant peanut allergen despite similarities with Ara h 6
https://doi.org/10.1016/j.jaci.2020.03.026
Abstract
Background
Ara h 2 specific-IgE (sIgE) is to date the best serologic marker to diagnose peanut allergy. Ara h 6 shares ∼60% sequence identity and multiple epitopes with Ara h 2.
Objectiv
To assess the diagnostic utility and relative importance of Ara h 2 and Ara h 6 in peanut allergy.
Methods
A cohort (n=100) of peanut allergic (PA), peanut sensitized but tolerant (PS) and non-sensitized non-allergic (NA) children were studied. sIgE levels to peanut and individual allergens were quantified using ImmunoCAP. ImmunoCAP inhibition experiments and mast-cell activation tests (MAT) were performed to both Ara h 2 and Ara h 6. Statistical analyses were performed using SPSS v14 and Prism v7.
Results
Ara h 2-sIgE and Ara h 6-sIgE showed the greatest diagnostic accuracy for peanut allergy compared with sIgE to peanut and other peanut allergens. Most PA patients were sensitized to both Ara h 2 and Ara h 6. Ara h 2 reduced Ara h 2-sIgE binding more than Ara h 6 (p<0.001) whereas Ara h 6-sIgE binding was inhibited to a similar degree by Ara h 2 and Ara h 6 (p=0.432). On the MAT, Ara h 2 induced significantly greater maximal reactivity (p=0.001) and lower EC50 (p=0.002) than Ara h 6 when testing co-sensitized individuals.
Conclusions
Ara h 2-sIgE and Ara h 6-sIgE provide the greatest accuracy for diagnosis peanut allergy. Ara h 2 is the dominant conglutin in peanut allergy in the UK, despite a degree of cross-reactivity with Ara h 6.
All authors:
OliverHemmings GeorgeDu ToitFRCPCH SuzanaRadulovic GideonLack FRCPCH Alexandra F.Santos
　　创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。