原标题：2S蛋白Ara h7.0201与其他蛋白Ara h7亚型相比具有独特的表位，在嗜碱性细胞脱粒能力上与2S蛋白Ara h2、6具有可比性
① 2S白蛋白对花生过敏有良好的预测价值，第三个2s蛋白Ara h7鉴定出3个亚型，其变应原特性还未被阐明；② 用免疫印迹法检测15例DBPCFC确诊的花生过敏患者对重组Ara h 2.0201、Ara h 6.01及重组Ara h 7亚型的敏感性；③ 9例患者中进行嗜碱性粒细胞激活试验（BAT），以确定过敏原的IgE耦合能力；④ 病人对Ara h 7.0201的敏感性最高（80%），并且在诱导嗜碱性粒细胞脱粒方面Ara h 7.0201与Ara h 2.0201和6.01一样活跃；⑤Ara h7.0201与其它2种异构体相比，存在独特的表位。
Clinical & Experimental Allergy
2S protein Ara h 7.0201 has unique epitopes compared to other Ara h 7 isoforms and is comparable to 2S proteins Ara h 2 and 6 in basophil degranulation capacity
Background: Screening for specific IgE against 2S albumin proteins Ara h 2 and 6 has good positive predictive value in diagnosing peanut allergy. From the third 2S member Ara h 7, 3 isoforms have been identified. Their allergenicity has not been elucidated.
Methods: Sensitization of 15 DBPCFC-confirmed peanut-allergic patients to recombinant Ara h 2.0201, Ara h 6.01 and isoforms of recombinant Ara h 7 was determined by IgE immunoblotting strips. A basophil activation test (BAT) was performed in 9 patients to determine IgE-cross-linking capacities of the allergens. Sensitivity to the allergens was tested in 5 patients who were sensitized to at least 1 Ara h 7 isoform, by a concentration range in the BAT. 3D prediction models and sequence alignments were used to visualize differences between isoforms and to predict allergenic epitope regions.
Results: Sensitization to Ara h 7.0201 was most frequent (80%) and showed to be equally potent as Ara h 2.0201 and 6.01 in inducing basophil degranulation. Sensitization to Ara h 7.0201 together with Ara h 2.0201 and/or 6.01 was observed, indicating the presence of unique epitopes compared to the other 2 isoforms.Differences between the 3 Ara h 7 isoforms were observed in C-terminal cysteine residues, pepsin and trypsin cleavage sites and 3 single amino acid substitutions.
Conclusions: The majority of peanut-allergic patients are sensitized to isoform Ara h 7.0201, which is functionally as active as Ara h 2.0201 and 6.01. Unique epitopes are most likely located in the C-terminus or an allergenic loop region which is a known allergenic epitope region for Ara h 2.0201 and 6.01. Due to its unique epitopes and allergenicity, it is an interesting candidate to improve the diagnostic accuracy for peanut allergy.
S. M. Hayen
Simone Hayen, Department of Dermatology/Allergology, UMC Utrecht, Utrecht, The Netherlands
S. M. Hayen, A. M. Ehlers, C. F. den Hartog Jager, J. Garssen, E. F. Knol, A. C. Knulst, W. Suer, L. E. M. Willemsen, H. G. Otten