①在出生队列中，用微阵列过敏原的方法对儿童进行过敏原特异性IgE应答分析，已经获得了儿童中特异性IgE应答演变的详细信息。需要关于过敏原特异性IgG早期进化的高分辨率数据。②分析妊娠期、脐带血样本、母乳和婴儿出生后第一年的IgG对微阵列过敏原的反应性，目的是研究母亲的过敏原特异性IgG是否可以防止子代IgE致敏。③在瑞典99个家庭的出生队列中，对母亲血浆样本、脐带血、母乳及子代不同时间点血浆分析164微阵列过敏原(ImmunoCAP ISAC technology)的IgG反应性，母亲的血浆在怀孕后期采集, ,母乳的收集在分娩后2个月, 儿童血浆样本在6、12和60个月时采集。IgE对微排列过敏原的敏感性是在儿童5岁时测定的。④母亲血浆、脐带血和母乳中的过敏原特异性IgG反应谱之间有高度相关性。母亲过敏原特异性IgG在一些6个月大的儿童中持续存在。孩子的过敏原特异性IgG在6个月时产生，反映了过敏原暴露。5岁时对过敏原IgE敏感的儿童比不敏感的儿童具有更高的过敏原特异性IgG水平。在所有164种测试过敏原中，对过敏原血清IgG升高(>30 ISU)的母亲的孩子在5岁时对该过敏原没有IgE敏感性。⑤这是第一次详细分析了母亲及其子代早期在分子层面的IgG识别谱。母亲血浆和母乳及脐带血中过敏原特异性IgG的高反应性在孩子5岁时看起来具有防止过敏的作用。⑥在妊娠晚期的母亲体内和脐带血中高水平的过敏原特异性IgG似乎可以防止后代的过敏。这一发现对预防过敏有意义。
JACI Available online 25 January 2019
Maternal allergen-specific IgG may protect the child against allergic sensitization
The analysis of allergen-specific IgE responses in birth cohorts with micro-arrayed allergens has provided detailed information regarding the evolution of specific IgE responses in children. High resolution data regarding early development of allergen-specific IgG are needed.
To analyze IgG reactivity to micro-arrayed allergens in mothers during pregnancy, in cord blood samples, breast milk and in infants in the first years of life with the aim to investigate if maternal allergen-specific IgG may protect against IgE sensitization in the offspring.
Plasma samples from mothers during third trimester, cord blood, breast milk collected at 2 months after delivery, and plasma samples from children at 6, 12 and 60 months of age were analyzed for IgG reactivity to 164 micro-arrayed allergens (ImmunoCAP ISAC technology) in 99 families in the Swedish birth cohort ALADDIN. IgE sensitizations to micro-arrayed allergens were determined at 5 years of age in the children.
Allergen-specific IgG reactivity profiles in mothers, cord blood and breast milk were highly correlated. Maternal allergen-specific IgG persisted in some children at 6 months. Children´s allergen-specific IgG production occurred at 6 months and reflected allergen exposure. Children who were IgE-sensitized against an allergen at 5 years of age had significantly higher allergen-specific IgG levels than non-sensitized children. For all 164 tested allergens, children from mothers with elevated (>30 ISU) specific plasma IgG against an allergen had no IgE sensitizations against that allergen at 5 years of age.
This is the first detailed analysis of the molecular IgG recognition profile in mothers and their children in early life. High allergen-specific IgG reactivity in mother´s plasma and breast milk and in cord blood seemed to protect against allergic sensitization at 5 years of age.
High levels of allergen-specific IgG in mothers during third trimester and in cord blood seem to protect against allergic sensitization in the offsprings. This finding has implications for the prevention of allergy.
lChristianLupinekMDa+HeidrunHochwallnerPhDa+CatharinaJohanssonPhDbAxelMiePhDbEvaRiglerMD, PhDaAnnikaScheyniusMD, PhDbcJohanAlmMD, PhDbcRudolfValentaMDade