杭州浙大迪迅生物基因工程有限公司JACI：孕妇过敏原特异性IgG检测的意义
发布日期：2019-03-01
原标题：母亲体内的过敏原特异性IgG可保护孩子免受过敏
延伸阅读
JACI Available online 25 January 2019
https://doi.org/10.1016/j.jaci.2018.11.051
Abstract:
Abstract
Background
The analysis of allergen-specific IgE responses in birth cohorts with micro-arrayed allergens has provided detailed information regarding the evolution of specific IgE responses in children. High resolution data regarding early development of allergen-specific IgG are needed.
Objective
To analyze IgG reactivity to micro-arrayed allergens in mothers during pregnancy, in cord blood samples, breast milk and in infants in the first years of life with the aim to investigate if maternal allergen-specific IgG may protect against IgE sensitization in the offspring.
Methods
Plasma samples from mothers during third trimester, cord blood, breast milk collected at 2 months after delivery, and plasma samples from children at 6, 12 and 60 months of age were analyzed for IgG reactivity to 164 micro-arrayed allergens (ImmunoCAP ISAC technology) in 99 families in the Swedish birth cohort ALADDIN. IgE sensitizations to micro-arrayed allergens were determined at 5 years of age in the children.
Results
Allergen-specific IgG reactivity profiles in mothers, cord blood and breast milk were highly correlated. Maternal allergen-specific IgG persisted in some children at 6 months. Children´s allergen-specific IgG production occurred at 6 months and reflected allergen exposure. Children who were IgE-sensitized against an allergen at 5 years of age had significantly higher allergen-specific IgG levels than non-sensitized children. For all 164 tested allergens, children from mothers with elevated (>30 ISU) specific plasma IgG against an allergen had no IgE sensitizations against that allergen at 5 years of age.
Conclusion
This is the first detailed analysis of the molecular IgG recognition profile in mothers and their children in early life. High allergen-specific IgG reactivity in mother´s plasma and breast milk and in cord blood seemed to protect against allergic sensitization at 5 years of age.
Clinical implication
High levels of allergen-specific IgG in mothers during third trimester and in cord blood seem to protect against allergic sensitization in the offsprings. This finding has implications for the prevention of allergy.
All Authors:
lChristianLupinekMDa+HeidrunHochwallnerPhDa+CatharinaJohanssonPhDbAxelMiePhDbEvaRiglerMD, PhDaAnnikaScheyniusMD, PhDbcJohanAlmMD, PhDbcRudolfValentaMDade
——浙大迪迅 译
①在出生队列中，用微阵列过敏原的方法对儿童进行过敏原特异性IgE应答分析，已经获得了儿童中特异性IgE应答演变的详细信息。需要关于过敏原特异性IgG早期进化的高分辨率数据。②分析妊娠期、脐带血样本、母乳和婴儿出生后第一年的IgG对微阵列过敏原的反应性，目的是研究母亲的过敏原特异性IgG是否可以防止子代IgE致敏。③在瑞典99个家庭的出生队列中，对母亲血浆样本、脐带血、母乳及子代不同时间点血浆分析164微阵列过敏原(ImmunoCAP ISAC technology)的IgG反应性，母亲的血浆在怀孕后期采集, ,母乳的收集在分娩后2个月, 儿童血浆样本在6、12和60个月时采集。IgE对微排列过敏原的敏感性是在儿童5岁时测定的。④母亲血浆、脐带血和母乳中的过敏原特异性IgG反应谱之间有高度相关性。母亲过敏原特异性IgG在一些6个月大的儿童中持续存在。孩子的过敏原特异性IgG在6个月时产生，反映了过敏原暴露。5岁时对过敏原IgE敏感的儿童比不敏感的儿童具有更高的过敏原特异性IgG水平。在所有164种测试过敏原中，对过敏原血清IgG升高(>30 ISU)的母亲的孩子在5岁时对该过敏原没有IgE敏感性。⑤这是第一次详细分析了母亲及其子代早期在分子层面的IgG识别谱。母亲血浆和母乳及脐带血中过敏原特异性IgG的高反应性在孩子5岁时看起来具有防止过敏的作用。⑥在妊娠晚期的母亲体内和脐带血中高水平的过敏原特异性IgG似乎可以防止后代的过敏。这一发现对预防过敏有意义。
延伸阅读
JACI Available online 25 January 2019
[IF:13.1]
Maternal allergen-specific IgG may protect the child against allergic sensitizationhttps://doi.org/10.1016/j.jaci.2018.11.051
Abstract:
Abstract
Background
The analysis of allergen-specific IgE responses in birth cohorts with micro-arrayed allergens has provided detailed information regarding the evolution of specific IgE responses in children. High resolution data regarding early development of allergen-specific IgG are needed.
Objective
To analyze IgG reactivity to micro-arrayed allergens in mothers during pregnancy, in cord blood samples, breast milk and in infants in the first years of life with the aim to investigate if maternal allergen-specific IgG may protect against IgE sensitization in the offspring.
Methods
Plasma samples from mothers during third trimester, cord blood, breast milk collected at 2 months after delivery, and plasma samples from children at 6, 12 and 60 months of age were analyzed for IgG reactivity to 164 micro-arrayed allergens (ImmunoCAP ISAC technology) in 99 families in the Swedish birth cohort ALADDIN. IgE sensitizations to micro-arrayed allergens were determined at 5 years of age in the children.
Results
Allergen-specific IgG reactivity profiles in mothers, cord blood and breast milk were highly correlated. Maternal allergen-specific IgG persisted in some children at 6 months. Children´s allergen-specific IgG production occurred at 6 months and reflected allergen exposure. Children who were IgE-sensitized against an allergen at 5 years of age had significantly higher allergen-specific IgG levels than non-sensitized children. For all 164 tested allergens, children from mothers with elevated (>30 ISU) specific plasma IgG against an allergen had no IgE sensitizations against that allergen at 5 years of age.
Conclusion
This is the first detailed analysis of the molecular IgG recognition profile in mothers and their children in early life. High allergen-specific IgG reactivity in mother´s plasma and breast milk and in cord blood seemed to protect against allergic sensitization at 5 years of age.
Clinical implication
High levels of allergen-specific IgG in mothers during third trimester and in cord blood seem to protect against allergic sensitization in the offsprings. This finding has implications for the prevention of allergy.
All Authors:
lChristianLupinekMDa+HeidrunHochwallnerPhDa+CatharinaJohanssonPhDbAxelMiePhDbEvaRiglerMD, PhDaAnnikaScheyniusMD, PhDbcJohanAlmMD, PhDbcRudolfValentaMDade
2019-1-30 Article
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