杭州浙大迪迅生物基因工程有限公司Allergy：广州市多螨过敏性哮喘患者过敏原特异性IgE分子成分的初步研究
发布日期：2023-02-10
原标题：广州市多螨过敏性哮喘患者过敏原特异性IgE分子成分的初步研究
方法:对南方某三级转诊中心57例多螨致敏的过敏性哮喘患者血清标本进行多重CRD特异性IgE检测，检测112种单一过敏原及其组分的特异性IgE。将结果与单次ImmunoCAP的结果进行比较。
结果：在ISAC中，nDer f 1（71.9%）、rDer f 2（73.7%）、nDer p 1（70.2%）和rDer p 2（66.7%）的致敏率最高，而rDer p 10和其他贮藏螨组分的阳性率仅为10%。rFel d 1和rCan f 1分别在29.8%和14.0%的样本中呈阳性。其他动物皮屑的阳性率均低于7.0%。对花粉组分的致敏主要为nCyn d 1（17.5%）和nPhl p 4（12.3%），4例nPhl p 4、nCyn a 2阳性、 nCyn d 1（CCD）阳性，均合并哮喘和鼻炎。对霉菌（rAsp f 3）、蟑螂（nBla g 7）和单线虫（rAni s 3）的敏感性相同，为8.8%。93.0%的患者对一种以上的组分敏感，其中有一半以上（57.9%）对五种或五种以上的组分呈阳性。合并哮喘和鼻炎患者（AA + AR）比仅哮喘患者（AA）对更多的组分敏感。AA + AR患者的nPhl p 4阳性率显著高于仅AA患者（χ2 = 4.31，P = 0.038）。
与ImmunoCAP相比，ISAC对屋尘螨和粉尘螨的检出率相当高，但热带无爪螨致敏患者汇中rBlo t 5的检出率仅为10.0%。过敏原组分相关性优化标度分析表明，rDer p10与食物过敏相关。
结论：本项目是第一个基于中国南方的多重芯片CRD研究，ISAC结果显示屋尘螨组分是导致哮喘患者过敏的主要过敏原组分。AA+AR合并患者相较于仅AA患者对更多组分敏感。其他阳性率较高的组分包括花粉组分nCyn d 1、nPhl P 4和动物皮屑组分rFel d 1和rCan f 1。ISAC中的rBlo t 5可能并不代表中国南方患者的热带无爪螨的主要致敏组分。
原始出处
molecular immunology
DOI: 10.1016/j.molimm.2018.11.004
Abstract:
Objective: Using multiplex microarray-based component resolved diagnosis (CRD) to investigate the allergen sensitization profile of allergic asthma patients in southern China.
Method: Serum samples from 57 polysensitized mite allergic asthmatic patients in a tertiary referral centre of southern China were tested with multiplex CRD (ISAC) for specific immunoglobulin E (sIgE) against 112 single allergen and components. Result was then compared with those from singleplex ImmunoCAP.
Results: With ISAC, the highest sensitization was seen for nDer f 1 (71.9%), rDer f 2 (73.7%), nDer p 1 (70.2%) and rDer p 2 (66.7%), whereas rDer p 10 and other storage mites’ components only showed 10% positivity. rFel d 1 and rCan f 1 were found positive in 29.8% and 14.0% samples respectively. Other epithelia components had less than 7.0% positive rate. Sensitization to pollen components was dominated by nCyn d 1 (17.5%) and nPhl p 4 (12.3%), Carbohydrate cross-reactive determinants (CCD) was positive in 4 patients who were also positive to nPhl p 4, nCyn d 1 and rPla a 2, and all of them have combined asthma and rhinitis. The sensitivity to mold (rAsp f 3), cockroach (nBla g 7) and Anisakis simplex component (rAni s 3) were all the same at 8.8%. 93.0% patients were sensitive to more than one component, with more than half of them (57.9%) positive to five or more components. Patients with combined asthma and rhinitis (AA+AR) were sensitive to more components than those with asthma only (AA). Positive rate to nPhl p 4 was significantly higher in patients with AA+AR than with AA only (χ2=4.31, P= 0.038).Compared with ImmunoCAP, ISAC showed a similar high detection rate for D. pteronyssinus and D. farinae, butonly 10.0% of B. tropicalis sensitive patients were positive to rBlo t 5. Optimal scale analysis on correlation of allergens components showed rDer p 10 was associated to food allergy.
Conclusion: Being the first multiplex microarray based CRD study on southern Chinese, ISAC showed house dust mites components were the major allergen components led to sensitization in asthmatic patients. Patients with combined AA+AR were sensitive to more components than those with AA only. Other components with higher positive rate include pollen components nCyn d 1, nPhl P 4 and animal dander components rFel d 1 and rCan f 1. For B. tropicalis, the rBlo t 5 in ISAC may not represent the major Blomia component in southern Chinese patients.
First Author:
Haisheng Hu
Correspondence:
State Key Laboratory of Respiratory Disease, First, Affiliated Hospital of Guangzhou Medical University, 151 Yanjiangxi Road, Guangzhou, 510120, Guangdong, China.
2023-02-10 Article
——浙大迪迅译
目的：采用多重微阵列组分诊断（CRD）方法，研究中国南方过敏性哮喘患者的过敏原致敏情况。方法:对南方某三级转诊中心57例多螨致敏的过敏性哮喘患者血清标本进行多重CRD特异性IgE检测，检测112种单一过敏原及其组分的特异性IgE。将结果与单次ImmunoCAP的结果进行比较。
结果：在ISAC中，nDer f 1（71.9%）、rDer f 2（73.7%）、nDer p 1（70.2%）和rDer p 2（66.7%）的致敏率最高，而rDer p 10和其他贮藏螨组分的阳性率仅为10%。rFel d 1和rCan f 1分别在29.8%和14.0%的样本中呈阳性。其他动物皮屑的阳性率均低于7.0%。对花粉组分的致敏主要为nCyn d 1（17.5%）和nPhl p 4（12.3%），4例nPhl p 4、nCyn a 2阳性、 nCyn d 1（CCD）阳性，均合并哮喘和鼻炎。对霉菌（rAsp f 3）、蟑螂（nBla g 7）和单线虫（rAni s 3）的敏感性相同，为8.8%。93.0%的患者对一种以上的组分敏感，其中有一半以上（57.9%）对五种或五种以上的组分呈阳性。合并哮喘和鼻炎患者（AA + AR）比仅哮喘患者（AA）对更多的组分敏感。AA + AR患者的nPhl p 4阳性率显著高于仅AA患者（χ2 = 4.31，P = 0.038）。
与ImmunoCAP相比，ISAC对屋尘螨和粉尘螨的检出率相当高，但热带无爪螨致敏患者汇中rBlo t 5的检出率仅为10.0%。过敏原组分相关性优化标度分析表明，rDer p10与食物过敏相关。
结论：本项目是第一个基于中国南方的多重芯片CRD研究，ISAC结果显示屋尘螨组分是导致哮喘患者过敏的主要过敏原组分。AA+AR合并患者相较于仅AA患者对更多组分敏感。其他阳性率较高的组分包括花粉组分nCyn d 1、nPhl P 4和动物皮屑组分rFel d 1和rCan f 1。ISAC中的rBlo t 5可能并不代表中国南方患者的热带无爪螨的主要致敏组分。
原始出处
molecular immunology
[IF：3.641]
A pilot study on the allergen-specific IgE to molecular components on polysensitized mite allergic asthmatic patients in Guangzhou, ChinaDOI: 10.1016/j.molimm.2018.11.004
Abstract:
Objective: Using multiplex microarray-based component resolved diagnosis (CRD) to investigate the allergen sensitization profile of allergic asthma patients in southern China.
Method: Serum samples from 57 polysensitized mite allergic asthmatic patients in a tertiary referral centre of southern China were tested with multiplex CRD (ISAC) for specific immunoglobulin E (sIgE) against 112 single allergen and components. Result was then compared with those from singleplex ImmunoCAP.
Results: With ISAC, the highest sensitization was seen for nDer f 1 (71.9%), rDer f 2 (73.7%), nDer p 1 (70.2%) and rDer p 2 (66.7%), whereas rDer p 10 and other storage mites’ components only showed 10% positivity. rFel d 1 and rCan f 1 were found positive in 29.8% and 14.0% samples respectively. Other epithelia components had less than 7.0% positive rate. Sensitization to pollen components was dominated by nCyn d 1 (17.5%) and nPhl p 4 (12.3%), Carbohydrate cross-reactive determinants (CCD) was positive in 4 patients who were also positive to nPhl p 4, nCyn d 1 and rPla a 2, and all of them have combined asthma and rhinitis. The sensitivity to mold (rAsp f 3), cockroach (nBla g 7) and Anisakis simplex component (rAni s 3) were all the same at 8.8%. 93.0% patients were sensitive to more than one component, with more than half of them (57.9%) positive to five or more components. Patients with combined asthma and rhinitis (AA+AR) were sensitive to more components than those with asthma only (AA). Positive rate to nPhl p 4 was significantly higher in patients with AA+AR than with AA only (χ2=4.31, P= 0.038).Compared with ImmunoCAP, ISAC showed a similar high detection rate for D. pteronyssinus and D. farinae, butonly 10.0% of B. tropicalis sensitive patients were positive to rBlo t 5. Optimal scale analysis on correlation of allergens components showed rDer p 10 was associated to food allergy.
Conclusion: Being the first multiplex microarray based CRD study on southern Chinese, ISAC showed house dust mites components were the major allergen components led to sensitization in asthmatic patients. Patients with combined AA+AR were sensitive to more components than those with AA only. Other components with higher positive rate include pollen components nCyn d 1, nPhl P 4 and animal dander components rFel d 1 and rCan f 1. For B. tropicalis, the rBlo t 5 in ISAC may not represent the major Blomia component in southern Chinese patients.
First Author:
Haisheng Hu
Correspondence:
State Key Laboratory of Respiratory Disease, First, Affiliated Hospital of Guangzhou Medical University, 151 Yanjiangxi Road, Guangzhou, 510120, Guangdong, China.
2023-02-10 Article
