①背景：蟑螂是室内过敏原的重要来源之一，可导致IgE敏化，并可发展为鼻炎和哮喘。②目的：进行蟑螂过敏原组份分析，确定与哮喘和鼻炎相关的过敏原、抗体水平和致敏模式。③方法：在两组蟑螂敏感的有哮喘和鼻炎（n = 19）、无哮喘和鼻炎（n = 28）的10岁儿童中检测总蟑螂过敏原及8种蟑螂过敏原组份特异性IgE、IgG、IgG4抗体。过敏原组份特异性抗体水平在吸附有蟑螂第1、2、4、5、6、7、9和11组重组过敏原的链霉亲和素ImmunoCAP上进行，而蟑螂特异性IgE用i6 ImmunoCap检测。④结果：有哮喘和鼻炎患儿和无哮喘和鼻炎患儿之间不同的是蟑螂过敏原组份和提取物特异性IgE水平，而不是IgG或IgG4。特别是，识别更多的蟑螂过敏原组份、更高的过敏原特异性IgE与疾病的发生相关。两组中均发现存在致敏模式的差异及无免疫显性过敏原的存在。过敏原组份特异性IgE的总和与总蟑螂过敏原（提取物）特异性IgE之间有良好的相关性(r = 0.86; p <0.001)。⑤结论：8种蟑螂过敏原组份分析显示，与哮喘和鼻炎的表现相关的IgE反应性具有显著的差异。过敏原特异性IgE的水平以及致敏谱与哮喘和鼻炎有关。
Cockroach allergen component analysis of children with or without asthma and rhinitis in an inner-city birth cohort
Cockroach is one of the most important sources of indoor allergens that can lead to IgE sensitization and to the development of rhinitis and asthma.
To perform a cockroach allergen component analysis to determine the allergens and antibody levels and patterns of sensitization that are associated with asthma and rhinitis.
Antibody levels (IgE, IgG and IgG4) to total cockroach and to eight cockroach allergens were determined in two groups of cockroach sensitized 10-year old children with (n = 19) or without (n = 28) asthma and rhinitis. Allergen-specific antibody levels were measured in streptavidin ImmunoCAPs loaded with each of the recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9, and 11, and total cockroach-specific IgE was measured using the i6 ImmunoCap.
IgE antibody levels to cockroach allergens and extract, but not IgG or IgG4, differed between subjects with and without asthma and rhinitis. Specifically, recognition of more cockroach allergens, with higher allergen-specific IgE, were associated with disease. Variable patterns of sensitization, with no immunodominant allergens, were found in both groups. There was a good correlation between the sum of allergen-specific IgE and total cockroach IgE (r = 0.86; p <0.001).
Component analysis of eight cockroach allergens revealed significant differences in IgE reactivity associated with the presence of asthma and rhinitis. Allergen-specific IgE titers and sensitization profiles were associated with asthma and rhinitis.
Anna Pomés Jill Glesner Agustin Calatroni Cindy M. Visness Robert A. Wood George T. O’Connor Meyer Kattan Leonard B. Bacharier Lisa M. Wheatley James E. Gern William W. Busse
· Genome Institute of Singapore, Agency for Science, Technology and Research Singapore, Singapore