杭州浙大迪迅生物基因工程有限公司

　　原标题：Alt a 1、真菌烯醇酶、锰依赖型超氧化物歧化酶(MnSOD)在孢菌科过敏的组份诊断中的作用
　　①背景：在过去的几十年里，基因组学和蛋白质组学对目前有关个性化过敏原组份的知识及其在诊断IgE介导的过敏方面的潜在应用做出了贡献。最近的研究表明，Alt a1被认为是孢子菌属的相关过敏原，烯醇化酶是真菌交叉反应的主要过敏原。然而，这些过敏原作为诊断变应原过敏的工具的真正用途仍不清楚。②目的：展示现有真菌过敏原系列组份的价值，以及建立准确的霉菌过敏原阵列以诊断对孢菌科过敏的必要性。③方法：在30位链格孢属过敏患者和100位捐献者的血液中，用ImmunoCAP™系统检测单个真菌过敏原和真菌过敏原提取物的特异性IgE。交叉反应研究采用单个过敏原和过敏原提取物的荧光酶免疫法(FEIA)和FEIA抑制法。用双向电泳结合MALDI-TOF分析的方法识别新的过敏原分子。④结果：所有过敏患者对不同分类学的几种霉菌均有阳性的特异性IgE反应。经典和分子诊断显示23%的患者有多种致敏。目前市面上的真菌过敏原阵列还不足以建立准确的诊断。链格孢属或Alt a1与弯曲菌或枝孢杆菌之间存在出乎意料的相关性，这激发了对更准确过敏原组份系列的研究。与Asp f6同源的锰依赖型超氧化物歧化酶(MnSOD)被鉴定为一种新的链格孢霉IgE结合分子。⑤结论和临床相关： Alt a1是对孢菌科过敏的标记物，不包括弯菌属。锰依赖型超氧化物歧化酶(MnSOD)可以在对Alt a 1不敏感的患者中解释6.6%的链格孢属过敏，在诊断孢菌科过敏时应与Alt a1和真菌烯醇化酶一起纳入分子阵列中。
延伸阅读
Allergy                                             
[IF:6.05]
Volume41, Issue3  March  2011  Pages 443-451
 Diagnostic value of Alt a 1, fungal enolase and manganese‐dependent superoxide dismutase in the component‐resolved diagnosis of allergy to pleosporaceae
doi.org/10.1111/j.1365-2222.2010.03671.x
Abstract:
Summary
 Background Over the last decades, genomics and proteomics have contributed to the current knowledge of individualized allergenic components and their potential use in the diagnosis of IgE‐mediated allergies. Recent investigations have demonstrated that Alt a 1 should be considered as a relevant allergen of the Pleosporaceae group and that enolase is the main allergen involved in the cross‐reactivity to fungi. However, the real utility of these allergens as tools for the diagnosis of allergy to Alternaria is still unclear.
Objective To demonstrate the current value of the available fungal allergen panel and the need to build an accurate mould allergen array for the diagnosis of allergy to Pleosporaceae.
Methods Specific IgEs to individual mould allergens and allergenic mould extracts were evaluated using the ImmunoCAP™ system in 30 patients allergic to Alternaria and in 100 blood donors. Cross‐reactivity studies were performed by Fluoro Enzyme ImmunoAssay (FEIA) and FEIA inhibition using individual allergens and allergenic extracts. Two‐dimensional electrophoresis associated with a MALDI‐TOF analysis was carried out to identify new allergen molecules.
Results
All allergic patients had positive specific IgE responses to several moulds from different taxonomical families. Classic and molecular diagnosis demonstrated that 23% of patients had multi‐sensitization. The current commercially available fungal allergen array was not sufficient to establish an accurate diagnosis. Unexpected correlations between Alternaria or Alt a 1 and Curvularia or Cladosporium stimulated the investigation of a more accurate allergen panel. A manganese‐dependent superoxide dismutase (MnSOD) homologous to Asp f 6 was identified as a new IgE‐binding molecule from Alternaria alternata.
Conclusions and Clinical Relevance Alt a 1 is the marker for allergy to Pleosporaceae, not including Curvularia. MnSOD can explain 6.6% of allergy to Alternaria without Alt a 1 sensitization and should be included together with Alt a 1 and fungal enolase in the molecular array for the diagnosis of allergy to Pleosporaceae
All Author:
I. Postigo  A. Gutiérrez‐Rodríguez J. Fernández J. A. Guisantes E. Suñén J. Martínez
　　创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。